The piRNA pathway is a piRNA-guided retrotransposon silencing system which includes processing of retrotransposon transcripts by PIWI-piRNAs in secondary piRNA biogenesis. Although several proteins participate in the piRNA pathway, the ones crucial for the cleavage of target RNAs by PIWI-piRNAs have not been identified. Here, we show that GTSF1, an essential […]
Yoshimura, T., Watanabe, T., Kuramochi-Miyagawa, S., Takemoto, N., Shiromoto, Y., Kudo, A., Kanai-Azuma, M., Tashiro, F., Miyazaki, S., Katanaya, A., Chuma, S., Miyazaki, J.-i.
Autophagy begins with the formation of autophagosomes, a process that depends on the activity of the serine/threonine kinase ULK1 (hATG1). Although earlier studies indicated that ULK1 activity is regulated by dynamic polyubiquitination, the deubiquitinase involved in the regulation of ULK1 remained unknown. In this study, we demonstrate that ubiquitin-specif […]
Kim, J. H., Seo, D., Kim, S.-J., Choi, D. W., Park, J. S., Ha, J., Choi, J., Lee, J.-H., Jung, S. M., Seo, K.-W., Lee, E.-W., Lee, Y. S., Cheong, H., Choi, C. Y., Park, S. H.
Valosin-containing protein (VCP) is an evolutionarily conserved ubiquitin-dependent ATPase that mediates the degradation of proteins through the ubiquitin–proteasome pathway. Despite the central role of VCP in the regulation of protein homeostasis, identity and nature of its cellular substrates remain poorly defined. Here, we combined chemical inhibition of […]
Heidelberger, J. B., Voigt, A., Borisova, M. E., Petrosino, G., Ruf, S., Wagner, S. A., Beli, P.
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent wi […]
Bozatzi, P., Dingwell, K. S., Wu, K. Z., Cooper, F., Cummins, T. D., Hutchinson, L. D., Vogt, J., Wood, N. T., Macartney, T. J., Varghese, J., Gourlay, R., Campbell, D. G., Smith, J. C., Sapkota, G. P.
SORCS1 and SORCS3 are two related sorting receptors expressed in neurons of the arcuate nucleus of the hypothalamus. Using mouse models with individual or dual receptor deficiencies, we document a previously unknown function of these receptors in central control of metabolism. Specifically, SORCS1 and SORCS3 act as intracellular trafficking receptors for tro […]
Subkhangulova, A., Malik, A. R., Hermey, G., Popp, O., Dittmar, G., Rathjen, T., Poy, M. N., Stumpf, A., Beed, P. S., Schmitz, D., Breiderhoff, T., Willnow, T. E.
Trafficking of mammalian ATG9A between the Golgi apparatus, endosomes and peripheral ATG9A compartments is important for autophagosome biogenesis. Here, we show that the membrane remodelling protein SNX18, previously identified as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes. ATG9A is recruited to SNX18-induced tubu […]
Soreng, K., Munson, M. J., Lamb, C. A., Bjorndal, G. T., Pankiv, S., Carlsson, S. R., Tooze, S. A., Simonsen, A.
The intracellular functions of myosin motors requires a number of adaptor molecules, which control cargo attachment, but also fine-tune motor activity in time and space. These motor–adaptor–cargo interactions are often weak, transient or highly regulated. To overcome these problems, we use a proximity labelling-based proteomics strategy to map the interactom […]
Activation of brown adipose tissue (BAT) and beige fat by cold increases energy expenditure. Although their activation is known to be differentially regulated in part by hypothalamus, the underlying neural pathways and populations remain poorly characterized. Here, we show that activation of rat-insulin-promoter-Cre (RIP-Cre) neurons in ventromedial hypothal […]
Wang, B., Li, A., Li, X., Ho, P. W., Wu, D., Wang, X., Liu, Z., Wu, K. K., Yau, S. S., Xu, A., Cheng, K. K.
Mutations in PINK1 cause autosomal recessive Parkinson's disease (PD), a neurodegenerative movement disorder. PINK1 is a kinase that acts as a sensor of mitochondrial damage and initiates Parkin-mediated clearance of the damaged organelle. PINK1 phosphorylates Ser65 in both ubiquitin and the ubiquitin-like (Ubl) domain of Parkin, which stimulates its E3 […]
The race to become crowned as the World’s Most Unloved Species was hotly contested, once again, this year with 19 nominated species in the running. After 12 days of fierce competition, impassioned pitches and over 4,500 votes, the top 10 was announced on Valentine’s Day. But slithering into first place… it’s the Galapagos racer! Often […]
Species name: thorny skate Nominated by: Shark Advocates International IUCN Red List classification: Vulnerable What is so special about your species? Thorny skates have amazing features, support substantial fisheries, and face serious threats. Yet, they get so little love. This fierce-looking, bottom-dwelling species has a dozen or more large thorns running […]
Species name: goliath frog Nominated by: Synchronicity Earth IUCN Red List classification: Endangered What is so special about your species? The goliath frog is the world’s largest frog. It is known only from south-western Cameroon from the region of Nkongsamba, and south to Monte Alen in mainland Equatorial Guinea. It is generally found at low to […]
Species name: shortfin mako Nominated by: The Shark Trust IUCN Red List classification: Vulnerable; Critically Endangered – Mediterranean What is so special about your species? Sleek and fast, the shortfin mako is capable of reaching speeds of over 30mph, making it the fastest shark in the world. On top of this, it has the power to […]